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Following Matrigel polymerization for 10 min at 37 °C, advanced DMEM/F12 was supplemented with penicillin/streptomycin, 2 mM GlutaMAX, 10 mM HEPES, 1× B27, 1× N2 (Life Technologies), 1mM N-acetylcysteine (Sigma), and 10 nM gastrin I (Biogems)
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